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Membrane filtration, TSA plate count, 3d at 30-35°C
Recovery test with B.subtilis spike on products
Recovery efficiency + method suitability + 2 ID of dominants
Extraction, filtration, TSA incubation 3-5d at 30-35°C, aerobic conditions
Anaerobic incubation on TSA or FTM media, 5-7 days
Extraction/rinse, filtration, TSA 3-5d & SDA 5-7d incubation
Crystal violet/iodine/safranin staining of colonies
Growth promotion with 5 strains on TSA plates
Growth promotion test with C.albicans, A.brasiliensis on SDA