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Spectrophotometric detection at 405nm after alkaline extraction
Recovery study with hemoglobin spike at 3 levels
BCA assay after extraction in 1% Triton x100 solution
Recovery validation with BSA spike, 3 concentration levels
72h extraction at 37°C or 50°C, TOC measurement of extract
Recovery study with sucrose spike in extraction water
Luminescence detection after swab/rinse extraction
Fluorescent DNA substrate degradation assay
qPCR quantification with human-specific primers
qPCR amplification efficiency test with internal control
Fluorometric assay with RNA substrate degradation monitoring