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L929 cells exposed to extracts, XTT viability assay
MatTek EpiDerm™ reconstructed human epidermis model
L929 cells, 24h direct contact, morphology assessment
Agar overlay method with L929 cells, zone assessment
Bacterial reverse mutation assay with/without metabolic activation
Mammalian cell forward mutation assay with colony sizing
Direct/extract contact with RBCs, hemoglobin release
Mouse systemic injection, polar/non-polar extracts
Rabbit intradermal injection of polar/non-polar extracts
Rabbit eye irritation test (Draize method)
Guinea pig maximization test, polar/non-polar extracts
Rabbit skin application, 4h exposure, 72h observation
Rabbit pyrogen test, saline extract, temperature monitoring
Hamster cheek pouch or oral mucosa irritation test
GLP-compliant rabbit pyrogen test with saline extract
Surface elemental analysis at 3 locations, depth <10nm