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Water extraction 72h at 37°C, HS-GC-MS analysis
IPA extraction 72h at 37°C, GC-MS screening
Hexane extraction 72h at 37°C, GC-MS analysis
Water extraction 72h at 50°C, GC-MS screening
L929 cells exposed to extracts, XTT viability assay
L929 cells, 24h direct contact, morphology assessment
Agar overlay method with L929 cells, zone assessment
Extraction in DMF, GC-FID quantification
Ethylene glycol by GC-FID after extraction
Simulated use extraction, GC-FID headspace analysis
Additional extraction cycle for EO/ECH
Recovery validation with hydrocarbon standard, GC-MS method
Hexane extraction 24h, GC-MS quantification
72h extraction at 37°C or 50°C, TOC measurement of extract
Recovery study with sucrose spike in extraction water
Mouse systemic injection, polar/non-polar extracts
Rabbit intradermal injection of polar/non-polar extracts
Guinea pig maximization test, polar/non-polar extracts
Rabbit skin application, 4h exposure, 72h observation
Additional element quantification by ICP-MS
Quantitative analysis for single element after extraction
Water extraction, semi-quantitative scan for 40 elements
Hexane extraction, LC-MS analysis
Water extraction, LC-MS screening for non-volatiles
IPA extraction, LC-MS non-volatile screening