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L929 cells exposed to extracts, XTT viability assay
MatTek EpiDerm™ reconstructed human epidermis model
L929 cells, 24h direct contact, morphology assessment
Agar overlay method with L929 cells, zone assessment
Extraction in DMF, GC-FID quantification
Bacterial reverse mutation assay with/without metabolic activation
Mammalian cell forward mutation assay with colony sizing
Direct/extract contact with RBCs, hemoglobin release
Mouse systemic injection, polar/non-polar extracts
Rabbit intradermal injection of polar/non-polar extracts
Rabbit eye irritation test (Draize method)
Guinea pig maximization test, polar/non-polar extracts
Rabbit skin application, 4h exposure, 72h observation
Rabbit pyrogen test, saline extract, temperature monitoring
Mouse systemic toxicity per USP Class VI requirements
Rabbit intracutaneous reactivity test with extracts
Rabbit muscle implantation, histopathology evaluation
Hamster cheek pouch or oral mucosa irritation test
GLP-compliant rabbit pyrogen test with saline extract